Molecular Detection of Rabies by SYBR Green Real Time PCR
نویسندگان
چکیده
منابع مشابه
Molecular Detection of Rabies by SYBR Green Real Time PCR
The present study was envisaged to compare the sensitivity of SYBR Green real time PCR with immunofluorescence PCR for diagnosis of rabies. SYBR Green real time PCR technique was applied on brain samples collected from 39 rabies suspected animals. Sensitivity of SYBR Green technique was compared in accordance with WHO recommended gold standard test viz. Fluorescent Antibody Technique (FAT) appl...
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The use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the da...
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Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
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ژورنال
عنوان ژورنال: IOSR Journal of Agriculture and Veterinary Science
سال: 2012
ISSN: 2319-2372,2319-2380
DOI: 10.9790/2380-0111618